Symbiotic Activity of Pea (Pisum sativum) after Application of Nod Factors under Field Conditions
نویسندگان
چکیده
Growth and symbiotic activity of legumes are mediated by Nod factors (LCO, lipo-chitooligosaccharides). To assess the effects of application of Nod factors on symbiotic activity and yield of pea, a two-year field experiment was conducted on a Haplic Luvisol developed from loess. Nod factors were isolated from Rhizobium leguminosarum bv. viciae strain GR09. Pea seeds were treated with the Nod factors (10⁻¹¹ M) or water (control) before planting. Symbiotic activity was evaluated by measurements of nitrogenase activity (acetylene reduction assay), nodule number and mass, and top growth by shoot mass, leaf area, and seed and protein yield. Nod factors generally improved pea yield and nitrogenase activity in the relatively dry growing season 2012, but not in the wet growing season in 2013 due to different weather conditions.
منابع مشابه
Optimum conditions for asparaginase extraction from Pisum sativum subspp. Jof. Zena Abdulla Khalaf, Nabeel Khalaf Al-Ani* and Hameed Majeed Jasim
Asparaginase was extracted from plant parts of Pisum sativum subspp. Jof collected from a field crop. Asparaginase activity was detected in seeds, stems and leaves extracts. Enzyme activity was higher in seeds extracts (30.0 U/ml) compared with leaves extracts (26.4 U/ml) and stems extracts (16.1 U/ml), respectively. Optimum conditions for the activity of crude asparaginase extracted from plant...
متن کاملاثر پرایمینگ بذر بر برخی خصوصیات فیزیولوژیکی مؤثر بر جوانهزنی بذر نخودفرنگی (Pisum sativum L.) تحت تنش سرما
In order to evaluate the effect of seed priming on some effective physiological parameters on seed germination of pea (Pisum sativum L.) under chilling stress, a factorial experiment was conducted in completely randomized design with four replications. Treatments were consisted of priming treatments (hydropriming, halopriming, osmopriming and no priming) and temperature (3, 6, 9, 12 and 15 ˚c)....
متن کاملNod factors induce nod factor cleaving enzymes in pea roots. Genetic and pharmacological approaches indicate different activation mechanisms.
Establishment of symbiosis between legumes and rhizobia requires bacterial Nod factors (NFs). The concentration of these lipochitooligosaccharides in the rhizosphere is influenced by plant enzymes. NFs induce on pea (Pisum sativum) a particular extracellular NF hydrolase that releases lipodisaccharides from NFs from Sinorhizobium meliloti. Here, we investigated the ability of non-nodulating pea...
متن کاملEffect of Naringein and Quercetin on Activity of Nodabc Genes of Strain D293 and following Nodulation and Nitrogen Fixation Response of Inoculated Pea Plants (pisum Sativum L.)
Concentration and time course dependence of two structurally related plant flavonoids naringenin and quercetin on the nodABC gene transcriptional activity (as reporter gene nodC-lacZ activity) in Rh. leguminiosarum bv. vicae strain D923 and respective growth, nodulation and nitrogen fixing responses of plant host (garden pea) to inoculation with pre-induced strains were studied. Naringenin was ...
متن کاملEffect of Fungicides and Bioinoculants on Pisum sativum
Fungicides seed dressing provides benefits by preventing fungal infection, conversely, damage the non-target organisms including plant and its symbiotic bacteria. In this study, the effect of two fungicides, carbendazim and thiophanate methyl, was assessed on the growth of pea and its symbiotic bacteria, Rhizobium leguminosarum. In vitro studies revealed that recommended concentration of fungic...
متن کامل